Fig. 5: PICS promoting 21U RNAs biogenesis are dependent of the interactions of PICS subunits.
From: Molecular basis for PICS-mediated piRNA biogenesis and cell division
a Images of adult germline cells expressing GFP tagged-TOFU-6 or TOFU-6 D26A/W27A in indicated mutant backgrounds. TOFU-6 D26A/W27A impairs the interaction between TOFU-6 and PICS-1(PID-3). b Images of adult germline cells expressing GFP tagged- PICS-1(PID-3) in indicated mutant backgrounds. c Images of adult germline cells expressing GFP tagged wild-type or amino acids- substituted PICS-1(PID-3) in pics-1 (tm2417) background. K245A/F246A and Y250A/Q251A of PICS-1(PID-3) weaken the interaction with TOFU-6 . F217E disrupts the homodimerization of PICS-1(PID-3). I182D/V186D and V189D/L190D of PICS-1(PID-3) disrupt the interaction with ERH-2. d Images of adult germline cells, oocytes and embryos expressing GFP tagged ERH-2 in indicated mutant backgrounds. e Images of adult germline cells, oocytes and embryos expressing GFP tagged wild-type or amino acids- substituted ERH-2 in erh-2 null mutant background. ERH-2 M38D disrupts the interaction with PICS-1(PID-3) . ERH-2 D67A disrupts the interaction with TOST-1. f Images of adult germline cells, oocytes and embryos expressing GFP tagged TOST-1 in indicated mutant backgrounds. g Images of adult germline cells, oocytes and embryos expressing GFP tagged wild-type or amino acids- substituted TOST-1 in tost-1 null mutant background. L39A/F43A and R42C of TOST-1 disrupt the interaction with ERH-2. Scale bars, 20 μm. The worms were harvested once, while the imaging experiments were triplicated.
