Fig. 2: Difference of MASCp36 cellular tropism in lungs from 9-month-old and 8-week-old mice.
From: Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2
a Multiplex immunofluorescence staining for detection of SARS-CoV-2 targeted cells in lung sections of male mouse (9-month-old) at 1 dpi. SARS-CoV-2 N protein (red), ACE2 (white), SPC (green), PDPN (gold), CC10 (magenta), FOXJ1 (cyan). The framed areas are shown adjacently at a higher magnification. The yellow arrows indicate ACE2+ club cells infected with SARS-CoV-2, and the red arrows indicate decreased ACE2 expression in infected cells (n = 3 per group). b Multiplex immunofluorescence staining of lung sections for SARS-CoV-2 N protein (red) and SPC (green) detection in male mice at 1 dpi (n = 3 per group). c Multiplex immunofluorescence staining for detection of ACE2 (white) and SPC (green) expression in lung tissues from the uninfected control mice (9-month-old and 8-week-old). The framed areas are shown below at a higher magnification. The yellow arrows indicate ACE2+ cells. The percentage of ACE2+ cells in the SPC+ AT2 compartment was statistically analyzed by two-tailed Student’s t test (P = 0.0002) ***P < 0.01. Data are presented as mean ± SD (n = 3 per group).
