Fig. 6: MLKL-mediated necroptosis triggers skin inflammation in OTULINE-KO mice.
From: OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice
a Kaplan–Meier plot depicting survival of mice of the indicated genotypes. b Graph depicting macroscopic skin score of mice of the indicated genotypes. The dots in the graphs represent individual mice. Mean ± s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Kruskal–Wallis test (one-sided). c Representative images of skin sections from 3-week-old mice of the indicated genotypes, stained with H&E (n = 4) or immunostained with anti-CC3 (n = 3), anti-CC8 (n = 3) or anti-Ki-67 (n = 3) antibodies. Scale bars: H&E = 100 μm; CC3, CC8, Ki-67 = 50 μm. d Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 4), anti-K6 (n = 3), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm. e Graphs depicting relative mRNA expression of the indicated genes in RNA from whole-skin tissue of 3-week-old mice of the indicated genotypes, measured by qRT-PCR. The dots in the graphs represent individual mice. Mean ± s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Kruskal–Wallis test (one-sided). Control mice include littermates that do not express K14-Cre. Source data for a, b, e are provided as a Source data file.
