Fig. 1: Synthetic route for the preparation of chemical probes.
From: A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites
Synthesis of electrophilic (a) and photoaffinity probes (b, c). (1) PreQ1, K2CO3, DMF, rt, 1 h; (2) PreQ1,TEA, DMF, rt; (3) PreQ1, HATU, DIEA, DMF, 0 °C to rt 1 h; (4) PreQ1, TEA, DMF, rt, 18 h; (5) Ph3P, Imidazole, I2, DCM, 0 °C, 4 h; (6) PreQ1, K2CO3, DM rt, 2 h (in dark); (7) PreQ1, K2CO3, DMF, rt, 1 h; (9) Trifluoroacetic acid/DCM, 0 °C rt, 30 min; (8) PreQ1, K2CO3, DMF, rt, 2 h (in dark).
