Fig. 2: Biochemical optimization of photocrosslinking and competition experiments to evaluate the specificity of compound 11 for the Ss-preQ1-RS aptamer.
From: A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites
a Dose-dependent crosslinking efficiency of compound 11. b Time-dependent crosslinking efficiency of compound 11. Competition experiments in the presence of c PreQ1 and d tRNA. e PAGE analysis to assess crossreactivity of compound 11 with other structured RNAs shows no quantifiable modification of either miR-21 RNA or SAM-II RNA. Values for crosslinking efficiency are reported as the mean ± standard deviation of three replicate experiments. Source data are provided as a Source Data file.
