Fig. 7: Combined HGF and anti-PD-L1 immunotherapy inhibited melanoma metastasis effectively. | Nature Communications

Fig. 7: Combined HGF and anti-PD-L1 immunotherapy inhibited melanoma metastasis effectively.

From: A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis

Fig. 7

a C57BL/6 mice were intravenously (i.v.) injected with 1 × 105 B16F10-FLuc cells, treated with PBS, anti-PD-L1, HGF (day 6, day 8, and day 10), or HGF every 2 days from day six for three times, and anti-PD-L1 on day 7, day 9, and day 11 (HGF + anti-PD-L1). b Respective bioluminescence images of metastatic B16F10-FLuc cells in mice body on day 12, day 15, day 18, and day 21. c Representative photographs of tumor nodules in lungs on day 21. d Number statistics of tumor nodules on lungs on day 21 after treatment. e H&E-stained slice images of lungs. Scale bar: 400 μm. Data were presented as mean ± SD. n = 4 biologically independent animals per group. Statistically significant differences between groups were identified by one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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