Fig. 3: αCD47-G1 but not αCD47-G4 induces cytotoxicity of human NK cells against GBM cells. | Nature Communications

Fig. 3: αCD47-G1 but not αCD47-G4 induces cytotoxicity of human NK cells against GBM cells.

From: An oncolytic virus expressing a full-length antibody enhances antitumor innate immune response to glioblastoma

Fig. 3

a, b Cytotoxicity of human primary NK cells against αCD47-G1- and αCD47-G4-treated (a) GBM43 and (b) BT422 human GBM cells. c Cytotoxicity of human primary NK cells against GBM43 cells in control media or conditioned media from the culture of OV-Q1-, OV-αCD47-G1- or OV-αCD47-G4-infected U251T2 GBM cells. d The effect of αCD47-G1 or αCD47-G4 on the expression of the NK cell activation marker CD69 when cocultured with GBM43 cells at an effector:target ratio of 1:1. e Summary data of (d). All experiments were performed with three donors in triplicate. f The effect of αCD47-G1 and αCD47-G4 on granzyme B expression of NK cells when cocultured with GBM43 cells. g Summary data of (f). All experiments were performed with three donors in triplicate. Error bars represent standard deviations of means of three donors. For (e) and (g), one-way ANOVA with P values corrected for multiple comparisons by the Bonferroni test.

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