Fig. 1: Graphical overview of the nanopore protein fingerprinting approach.
Peptides are pre-hydrolyzed by a specific protease (e.g. trypsin) and the resulting peptides are measured as they translocate the nanopore. Each peptide entering the nanopore reduces the open pore current (Io) to the blocked pore current (IB). The resulting excluded current (ΔIB = Io − IB) relates to the volume of the peptide. The subsequent histogram of the percent of excluded currents (Iex %= ΔIB/ IO %) is used to identify the protein.
