Fig. 3: Protein identification using nanopore spectrometry.

a Baseline-corrected excluded current spectra of 9 tryptic digested proteins. Based on 3 individual measurements. b Leave-one-out spectral matching of the baseline corrected excluded current spectra using Euclidean cosine cross-correlation. With on the Y-axis the protein that is being matched using leave-one-out matching with the proteins on the X-axis. The black boxes represent the matches with the highest score. All nanopore measurements were performed in 1 M KCl buffered to pH 3.8 using 50 mM citric acid titrated with bis-tris-propane under an applied potential of −70 mV. Recording was performed at 50 kHz using an analog Bessel-filter at 10 kHz and a digital Gaussian filter of 5 kHz.