Fig. 6: PNRSS sensing of remdesivir and remdesivir triphosphate metabolite.

a The sensing mechanism. Remdesivir and the remdesivir metabolite both contain a ribose moiety, capable of binding to a PBA. b Representative events of remdesivir and remdesivir metabolite. The label R and M respectively marks remdesivir and remdesivir metabolite. The events were Butterworth low-pass filtered with a cut-off frequency of 100 Hz (Supplementary Fig. 44). Both events appear positive going (\({I}_{{{{{\mathrm{b}}}}}} \; > \; {I}_{{{{{\mathrm{p}}}}}}\)). Binding of remdesivir results in time extended events with characteristic noise fluctuations. Remdesivir metabolite on the other hand reports transient events with minimum noise. c A scatter plot of event amplitude SD against the event dwell time. The histogram of the event amplitude SD and its Gaussian fitting results were plotted to the right. Results of 119 events are included. d A scatter plot of high-pass (Hp) and low-pass (Lp) amplitude SD. Both analytes are clearly separated in the scatter plot. Results of 126 events are included. e A continuous trace containing binding events from remdesivir and remdesivir metabolite. The identity of each event is called based on event characteristics as described in d.