Fig. 1: Expression of trip6 in embryonic and postnatal brain. | Nature Communications

Fig. 1: Expression of trip6 in embryonic and postnatal brain.

From: TRIP6 functions in brain ciliogenesis

Fig. 1

a mRNA expression in trip6+/+ mouse brain sections by in situ hybridization (ISH) depicting embryo sagittal sections (E14.5–E18.5) and postnatal coronal sections (P0–P6). Pall pallium, M midbrain, Tel telencephalic vesicle, H hindbrain, ChP choroid plexus, Cb cerebellum, CTX cerebral cortex, CPu Caudate Putamen, aca anterior commissure, Pit pituitary, LV lateral ventricle, 3V third ventricle, Hip hippocampal formation. Choroid plexus locations within the lumen of the ventricles are indicated by arrows. Control sense ISH is shown in Supplementary Fig. 1a, b. Immunofluorescence labeling of TRIP6 in ependymal cells lining the 4th ventricle (b) and choroid plexus epithelium (c) demonstrates its co-localization with β-catenin (arrow) and ZO1 (arrowhead). Nuclei were stained with DAPI. The marked areas are magnified in the insets. Ages are indicated in the figure. For number of mice analyzed, please see “Methods” section. Asterisk (*) indicates the autofluorescence of the choroid plexus vasculature. Scale bars: 1 mm (a), 50 μm and 250 μm (b and magnified images, respectively), 10 μm (c).

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