Fig. 2: trip6 deletion causes perinatal hydrocephalus.

a Macroscopic view of cranial malformation indicative of hydrocephalus (arrow) in a trip6^−/− mouse (unaffected trip6^+/− littermate shown for comparison). b Quantification of hydrocephalus incidence in control and trip6^−/− mice. n number of mice assessed (p < 0.0001, two-tailed chi squared test; see also Methods and Supplementary Table 5). Source data are provided as a Source Data file. c H&E stained sagittal brain sections showing enlarged LVs in a trip6^−/− mouse (unaffected trip6^+/− littermate shown for comparison). d Coronal brain MRI images from control and hydrocephalic mice at the level of lateral (LV) and 3rd ventricle (3V) demonstrate the enlargement of LV, but not 3V, in the trip6^−/− mouse. In the colorized heat maps the fluid-filled ventricles appear yellow. e Lateral (“sagittal”) (L) and horizontal (H) brain MRI sections and lateral view of a maximum intensity projection (MIP) reconstruction depicting the ventricular system (white) in mice of the indicated genotypes (P21). Dashed lines (L and H) indicate the section planes of lateral and horizontal views, respectively. The LV is seen only in L40 and not in L36 in the wt. In control, LV, vs. 3V and the 4th ventricle (4V) can only be visualized in separate lateral sections (L40, L36) while the LV of trip6^−/− encompasses several sections (L31, L35). In contrast to LV, the 3V and 4V are not enlarged. The MIP views show the entire ventricular system. Note the massive expansion of the ventricular system in trip6^−/− compared to the heterozygote. Scale bar: 1 mm (c, d). For 3D reconstruction of the complete set of sections, please see Supplementary Movies 1 and 2.