Fig. 8: Downregulation of trip6 by siRNA or blocking of TRIP6 dimerization, in the choroid plexus-derived cell line Z310, impairs cilium elongation or ciliogenesis.

Z310 cells, transfected with the indicated siRNAs (siTrip6 or siControl) (a) or peptides (dimerization blocking or control) (e), were serum-starved to induce primary cilium formation (see “Methods” section for detailed description). The cells were analysed by immunofluorescence microscopy to visualize basal bodies (labeled by anti-γ-tubulin antibody) and the primary cilium (anti-ARL13B), and were counterstained with anti-TRIP6 and DAPI (a’ shows magnified cilia from panel a). Both inhibitory treatments significantly impaired primary cilium formation (b, f; analysed by two-tailed Fisher’s exact test) or—in those cells carrying cilia-like structures—cilium morphology (c, g; two-tailed Fisher’s exact test) and length (d, h; two-tailed Student’s t-test). Both phenotypes were adversely and significantly affected. The box plots (d, h) indicate the median (middle line), the interquartile range (IQR) from 1st to the 3rd quartile (box), and the upper edge (Q1 + 1.5*IQR) to lower edge (Q1 − 1.5*IQR) (whiskers). In the morphological assessment, “puncta” indicate basal bodies without associated axoneme. For detailed description of quantification and statistics, see “Methods” section. Source data are provided as a Source Data file. Additional analysis of the RNAi experiments is presented in Supplementary Fig. 5. Scale bars: 10 μm (a, a', e).