Fig. 9: Localization of TRIP6 in primary cilia of choroid plexus-derived Z310 cells, via super resolution microscopy. | Nature Communications

Fig. 9: Localization of TRIP6 in primary cilia of choroid plexus-derived Z310 cells, via super resolution microscopy.

From: TRIP6 functions in brain ciliogenesis

Fig. 9

Representative examples of six primary cilia, labeled with the structural cilia components acetylated α-tubulin (Ac.TUB), ARL13B or CLUAP, and with anti-TRIP6. TRIP6 localized in foci along the axoneme, exhibiting variable distribution among individual cilia (arrows in b, f) when compared to the more uniform sub-ciliary localization of Ac.TUB and ARL13B (a, b). Super resolution identifies two major axonemal sites of CLUAP (c). Additionally, TRIP6 partially co-localized with the basal body markers pericentrin (PCNT; a, d) and γ-tubulin (γ-TUB; b). Basal bodies are indicated by circles. Downregulation of trip6 expression, by siRNA, reduced the length of the axoneme without affecting the basal body (e cf. d). Nevertheless, those cells presenting with elongated cilia, in the siTrip6-treated cultures (partial downregulation), were found to still express TRIP6 that localized along the axoneme (arrows, f) and in the basal body (circle, f). Scale bars: 1 μm.

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