Fig. 2: Comparative transcriptome analysis of thymic B cell subsets and cord blood B cells.

a Principal component analysis (PCA) clustering of thymic CD21⁻CD35⁻ (red dots), CD21⁺CD35⁺ (blue dots) and cord blood (green dots) B cells using top 500 most variable genes (n = 5). b Heatmap of thymic CD19⁺CD21⁻CD35⁻ and CD19⁺CD21⁺CD35⁺ B cell subsets as well as cord blood CD19⁺ B cells after DESeq2 analysis pipeline. Data are expressed as normalized row z-score of log2 gene expression values (n = 5). Two-sided Wald test with Benjamini–Hochberg false discovery ration (FDR) adjustment. c Normalized counts of CD43 gene expression in thymic CD19⁺CD21⁻CD35⁻, CD19⁺CD21⁺CD35⁺, and cord blood CD19⁺ B cell subsets after DESeq2 analysis pipeline. Error bars are expressed as mean ± SD (n = 5); two-sided t-test. d Heatmap representation of expression of genes included in the canonical mouse B1 cell signature in human thymic CD21⁻CD35⁻, CD21⁺CD35⁺ CD19⁺ B cell subsets as well as human cord blood CD19⁺ cells. Data are expressed as normalized row z-score of log2 gene expression values (n = 5). Two-sided Wald test with Benjamini–Hochberg false discovery ration (FDR) adjustment.