Fig. 5: Intrathymic differentiation of thymic plasma cells in human neonates.

a RNA velocity analysis of scRNA-seq data from thymic B cells and predicted cluster trajectory. b Expression of immunoglobulins in thymic individual B cells located mainly in Cluster 4 and colored by intensity from gray (no expression) to dark red (the highest expression). c Representative FACS plots of CD138⁺ plasma cells after in vitro culture of cord blood (top) and neonatal thymic (bottom) CD70⁺CD138⁺ B cells using conditions to promote differentiation into plasma cells. d Frequency of CD138⁺ plasma cells (%) pre and post-culture of CD70⁺CD138⁻ B cells isolated from cord blood (n = 3) or neonatal thymus specimens (n = 6). Two-sided t-test was performed.