Fig. 2: The mutation spectrum in Z. tritici is affected by epigenetic modification and temperature.
From: Epigenetic modifications affect the rate of spontaneous mutations in a pathogenic fungus
a Rates of base substitution mutations according to transitions and transversions. Comparison between Z. tritici wild type strains (IPO323, Zt05, and Zt10), which differ for DIM2-mediated 5mC methylations (Zt10 shows DIM2-mediated 5mC methylations; IPO323 and Zt05 do not) and comparison between Z. tritici strains (IPO323 ∆chr18, IPO323 ∆chr18 ∆kmt1, and IPO323 ∆chr18 ∆kmt6), which differ in the presence of histone modifications H3K9me3 and H3K27me3, respectively. In addition, the effect of an increase in temperature from 18 °C to 28 °C on the rate of base substitution mutations is depicted. G:C > A:T summarizes G → A and C → T base substitution mutations (analogous in the other depicted categories). Data were pooled for all replicated MA lines (n = 40 independently evolved MA lines for each strain) and is presented as mean values. Error bars represent 95% Poisson confidence intervals. b Equilibrium GC content estimated on the rates of mutations in AT direction (G:C > A:T transitions and G:C > T:A transversions) and the rate of the mutations in GC direction (A:T > G:C transitions and A:T > C:G transversions), compared to the observed GC content in the respective reference genomes. Data were pooled for all replicated MA lines (n = 40 independently evolved MA lines for each strain) and is presented as mean values. Error bars represent the SE as described in Supplementary Methods.
