Fig. 5: The removal of the histone modifications H3K9me3 and H3K27me3, and an increase in temperature affect the rate of mutations differently between different genomic compartments.
From: Epigenetic modifications affect the rate of spontaneous mutations in a pathogenic fungus
a Boxplot comparing the rate of base substitution rates in different compartments for all replicated MA lines of IPO323 ∆chr18 and IPO323 ∆chr18 ∆kmt1 (lacking H3K9me3), and IPO323 ∆chr18 ∆kmt6 (lacking H3K27me3) propagated at 18 °C for 52 weeks and IPO323 ∆chr18 propagated for 4 weeks at 28 °C (n = 40 independently evolved MA lines per strain). b Detailed analysis of the effect of the mutation rate in genomic compartments associated with TE, H3K9me3, and H3K27me3 using pooled data of all replicated MA lines for each strain. Effects of the removal of the respective histone modification and increase in temperature c on the relative frequency of transversions and transitions, d on whole chromosome aberrations, and e on structural variants. The genomic compartments are defined by the presence of the indicated genetic or epigenetic feature in the wild type IPO323. Categorized p-values of a FDR-adjusted two-sided Wilcoxon signed rank test or b–e two-sided Fisher’s exact test or FDR-adjusted two-sided χ2-test are depicted (*p < 0.05, **p < 0.005, ***p < 0.0005, ns = not significant at α = 0.05). The exact p-values for of all pairwise comparisons are provided in Supplementary Data 1. b, d, e Data were pooled for all replicated MA lines (n = 40) of each strain and is presented as mean values. Error bars depict 95% Poisson confidence intervals. a Box plots depict center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers.
