Fig. 3: Protease activity detected in supernatants of B. salamandrivorans zoospores.

Supernatants were collected by centrifuging the B. salamandrivorans zoospore suspensions treated with 50 mM D-galactose (Gal), 50 mM D-mannose (Man), H₂O and protease inhibitor mix (PI). Three technical replicates were performed per biological replicate, with three biological replicates in total. In the box-and-whisker plots, the median is shown as a line inside the box, the first and third quartiles shown as the lower and upper edges of the box, respectively, and the minimum and maximum values shown as whiskers. The significance of difference between the means is shown by *p = 0.036, **p = 0.008, ***p < 0.001; based on Tukey’s multiple comparisons test comparing two-sided linear mixed models. Source data are provided as a Source Data file.