Fig. 5: Extracellular vesicles isolated from the cell culture supernatant of activated CD73+ CD8 T cells are immunosuppressive. | Nature Communications

Fig. 5: Extracellular vesicles isolated from the cell culture supernatant of activated CD73+ CD8 T cells are immunosuppressive.

From: CD73-mediated adenosine production by CD8 T cell-derived extracellular vesicles constitutes an intrinsic mechanism of immune suppression

Fig. 5

CD73− CD4con T cells were stimulated with αCD3/αCD28 in the presence of the ADA inhibitor EHNA (10 µM) and incubated with AMP (50 µM), recombinant CD73 (15 ng/mL), EVs and PSB-14685 (10 µM) as indicated. a CD25 expression and b proliferation were measured after 4 days by flow cytometry. Data were shown as a median of three donors from independent experiments. For each donor, CD25 expression and proliferation were set in relation to cells only treated with EHNA. Repeated measures one-way ANOVA with Dunnett’s multiple comparisons test was used to compare all conditions to cells treated with EHNA and AMP (second bar).

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