Fig. 7: Cys-FeS clusters continue to form over time under anoxic conditions.
From: Spontaneous assembly of redox-active iron-sulfur clusters at low concentrations of cysteine
1H NMR spectra showing a the effect of Fe on free cysteine in solution essentially removing the cysteine signal. b Cys-FeS clusters prepared with increasing cysteine concentrations leading to increased signal for Hα and Hβ of bound cysteine. c HCO3−-FeS clusters prepared with increasing NaHCO3 concentrations leading to increased signal for Hα and Hβ of bound cysteine. d 5 mM Cys-FeS clusters in anoxic conditions over 14 h showing an increase in cysteine incorporation, and e 5 mM Cys-FeS clusters in the presence of oxygen over 14 h showing a decrease in bound cysteine. All solutions were prepared at pH 9. Source data are provided as a Source data file.
