Fig. 2: Vaccination of Syrian hamsters with ChAdOx1 nCoV-19 prevents lower respiratory tract infection with SARS-CoV-2 VOC B.1.1.7.

a Relative weight upon intranasal challenge with 10⁴ TCID₅₀ of B.1.1.7. Shown is the geometric mean with a 95% confidence interval (CI). *p-value < 0.05, corrected for multiple comparisons using the Holm–Šidák correction. b Lung:body weight (BW) ratio (mg:g) of hamsters euthanized at 5 DPI. Line = median. c sgRNA viral load in lung tissue obtained at 5 DPI. Line = median. Dotted line = limit of detection. d Infectious SARS-CoV-2 titer in lung tissue obtained at 5 DPI. Line = median. Dotted line = limit of detection. e Percentage affected lung tissue per animal as determined via histology. Line = median. f Percentage of lung tissue positive for SARS-CoV-2 antigen per animal. Line = median. g Truncated violin plot of the area under the curve (AUC) analysis of shedding as measured by sgRNA analysis in swabs collected on 1–5 DPI. Dashed line = median. Dotted line = quartiles. Statistical significance determined via multiple student t-tests with an n of 10 biologically independent animals (a), or two-tailed Mann–Whitney test (b–e) with an n of 4 biologically independent animals and (g) with an n of 10 biologically independent animals. p-values for days 4, 5, 6, 7, 8, 9, and 10 are 0.001251, < 0.000001, < 0.000001, < 0.000001, < 0.000001, < 0.000001, and < 0.000001, respectively. V = ChAdOx1 nCoV-19 vaccinated; C = ChAdOx1 GFP vaccinated; Orange circle = Hamsters vaccinated with ChAdOx1 nCoV-19, Blue square = Hamsters vaccinated with ChAdOx1 GFP.