Fig. 3: Engineering of the NG-ABEmax variants.
From: Human cell based directed evolution of adenine base editors with improved efficiency
a Mapping the key residues for L1-60, L1-98 and L2-156 using EGFP-based reporter system. Editing efficiencies were quantified by flow cytometry. Error bars indicate mean ± s.d. (n = 3 independent experiments). b Boosted editing efficiencies of NG-ABEmax-KR at endogenous genomic sites. Error bars indicate mean ± s.d. (n = 3 independent experiments). c Summary of boosted editing efficiencies of NG-ABEmax-KR at different A base at the EGFP sites. Error bars indicate mean ± s.d. (n = 3 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student’s unpaired two-sided t test. Exact P value of A1 = 0.002027 (A1 at 0.002027), A3 at 0.000347, A4 at 0.000167, A6 at 0.038443, A7 at 0.000012, A9 at 0.012894, A10 at 0.021788, A11 at 0.001507, A12 at 0.000910, A13 at 0.038361, A15 at 0.000149, A16 at 0.000345. d, e Increased editing window of NG-ABEmax-KR in site 8 and site 10. Each A base was highlighted in red. Error bars indicate mean ± s.d. (n = 3 independent experiments). Source data are available in the Source data file.
