Fig. 1: A 27 multiplex antibody panel to characterise the TME in mouse frozen tissues. | Nature Communications

Fig. 1: A 27 multiplex antibody panel to characterise the TME in mouse frozen tissues.

From: Characterisation of tumour microenvironment remodelling following oncogene inhibition in preclinical studies with imaging mass cytometry

Fig. 1: A 27 multiplex antibody panel to characterise the TME in mouse frozen tissues.

a Validation of antibodies in IMC using a tissue with known staining patterns, an example shown here is a follicle in the spleen; Ir191/193 (blue), B220 (green); CD4 (magenta), CD68 (yellow), αSMA (cyan), vimentin (red). Representative image of two independently stained spleen tissues. b Panel of 27 antibodies that identify multiple cell types from lymphoid, myeloid, tumour and stromal compartments, as well as markers of activation and proliferation status. Detailed information on the antibody clones and isotope conjugations can be found in Supplementary Tables 1 and 2. DC dendritic cells, NK natural killer, EMT epithelial-to-mesenchymal transition, Ag antigen.

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