Fig. 1: Schematic illustrations of histone imaging with plasmonic nanoprobes in a single cell. | Nature Communications

Fig. 1: Schematic illustrations of histone imaging with plasmonic nanoprobes in a single cell.

From: High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes

Fig. 1: Schematic illustrations of histone imaging with plasmonic nanoprobes in a single cell.

a Plasmonic nanoprobe conjugated with primary antibody of histone modification marker. Electron micrograph and dark-field scattering images of a single gold nanoparticle (GNP), images representative of five experiments. Black scale bar, 20 nm. White scale bar, 500 nm. b Representative dark-field scattering images of the plasmonic nanoprobes conjugated with antibody (Ab-GNP) targeting heterochromatin histone markers in a growing cell and a senescent cell. Images representative of five experiments. Scale bar, 10 µm. c An example of spatial arrangements of the plasmonic nanoprobes targeted to heterochromatic markers and simulated spectra of distance-dependent scattering of the probes. d Trends in the spectral peak (λmax) of simulated scattering according to the arrangement of plasmonic nanoprobes.

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