Fig. 2: Single histone marker (H3K9me3) imaging in OIS cells.
a Representative fluorescent image at each OIS time point using organic fluorophore conjugated with secondary antibody. Images, representing five experiments. Scale bar, 10 µm. b Comparison of the colors expressed by organic fluorophore and plasmonic nanoparticles according to the change in distance. c Conjugation strategy of primary antibody to 40 nm gold nanoparticle for preparing a plasmonic nanoprobe (Ab-GNP) with succinimidyl 6-[3′-(2-pyridyldithio)-propionamido] hexanoate (SPDP) linker. Images, representing five experiments. Black scale bar, 20 nm. White scale bar, 500 nm. d Change in absorbance before and after antibody conjugation to the gold nanoparticles. e Representative dark-field scattering images at each OIS time point. Images, representing five experiments. Scale bar, 10 µm. f Dark-field microscope setup for scattering imaging and spectral analysis of the plasmonic nanoprobes targeting histone marker. g λmax distributions of the spectra of scattering spots (n = 50) in single cells (n = 15 cells examined over five independent experiments) during OIS. Box plots indicate median (middle line), mean (□), 25th, 75th percentile (box), and range within 1.5 interquartile range (IQR, whiskers) as well as each data point (●). h Changes in colors (inset) and spectra of the scattering spots induced by the targeted assembly of the probes. i Time-lapse measurement of the maximum scattering wavelength (λmax) for the plasmonic nanoprobes in the nuclei after 4-OHT treatment (n = 15 cells examined over five independent experiments). Data are represented as mean ± s.e.m.
