Fig. 5: Correlation between orientation parameters of λ-DNA under different excitation polarization.
From: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function
a Super-resolution image of λ-DNA strands color coded with the same azimuthal map as Fig. 4a (scale bar is 3 μm). b Relative dipole orientations with respect to the DNA axis aligned along the x direction, illuminated without the QWP. On the left the view is aligned along the y axis, on the right is a top view aligned in the negative z direction. The dashed line highlights the primary population around Δϕ = 84°. c Relative dipole orientations of the same λ-DNA strand with a QWP in the illumination path. The dashed line highlights an additional population around Δϕ = 65°. Each bin spans 5° in the polar direction and 10∘ in the azimuthal direction, additionally there is a 30° coarse grid. Red arrows indicate the excitation polarization intensity. The distributions are similar on many different DNA-strands (N > 20) and samples (N > 10).
