Fig. 3: Immediate target genes of CBP/p300 identified by SLAM-seq.
a Schematic workflow of the SLAM-seq sample preparation protocol for the metabolic labelling of newly synthesized RNAs. MOLM-13 cells were treated with 10 µM C646 or DMSO, labelled with 4sU after 1 h, the RNA was collected after 2 h and subsequently sequenced. b Plots show the fold change (FC) in the abundance of total mRNA or newly synthesized mRNA in relation with their normalized baseline expression in reads per million (RPM). Genes significantly changed (FDR < 0.01) are highlighted in blue and red, respectively. c Top enrichment clusters of downregulated or d upregulated transcripts as determined by functional annotations clustering using DAVID68,69. e, f Relative expression of selected CBP/p300 target genes by RT-qPCR in MOLM-13 cells treated for 4 h with 10 µM C646 or 0.1% DMSO. g Relative expression of selected CBP/p300 target genes by RT-qPCR in MOLM-13 cells treated for 4 h with 1 µM A-485 or 0.01% DMSO. h Relative expression of selected CBP/p300 target genes by RT-qPCR in SKK-1 cells treated for 4 h with 10 µM C646 or 0.1% DMSO. c, d Statistical analysis was performed by Fisher’s exact test. e–h Data represent the mean ± SEM of at least three independent experiments. Statistical analysis was performed by two-sided Student’s T-test compared to untreated samples, *p-value < 0.05. Source data are provided as a Source Data file and in Supplementary Data 4 and 5.
