Fig. 2: Characterization of normal adjacent tissue derived pericytes and tumor-derived pericytes.

a Flow cytometry analysis of pericytes isolated from normal adjacent tissue and tumor-derived from NSCLC patients showed that the purity of pericyte preparation was high. Pericyte preparations displayed good expression of pericyte markers PDGFRβ, CD146, and CD13 and undetected or low level of endothelial cell markers CD31 and CD34 as well as immune cell marker CD45. b Immunofluorescent staining analysis of the endothelial cell, fibroblast, pericyte marker expression in NPC, TPC, HUVEC, and fibroblast (n = 3 independent samples). c RT-PCR analysis of the endothelial cell, fibroblast, smooth muscle cells, pericyte marker expression in NPC, TPC, HUVEC, fibroblasts, peripheral blood mononuclear cell (PBMC), smooth muscle cells (SMC) (for CD31, CD34, CD45, CNN1, MYOCD, and ACTA2 expression analysis: n = 4 independent NPC and TPC samples and n = 3 independent SMC, PBMC and HUVEC samples; for FAP, PDGFRA, PDGFRB, and CD146 expression analysis: n = 3 independent samples for each cell type). d Doubling time of NPC and TPC derived from NSCLC/HCC patients (n = 3 independent experiments). e β-galactosidase staining assay showed the percentage of senescent cells at different passages of NSCLC/HCC-derived NPC and TPC (n = 3 independent experiments). Statistical tests were two-sided. f, g Wound healing assay showed no difference in the migration ability of NSCLC/HCC-derived NPC and TPC (n = 3 independent experiments). Statistical tests were two-sided. Means ± SEM are given. c One-way ANOVA. d Two-way ANOVA. e–g Student’s t test. Scale bars in b represent 50 μm, e–g 100 μm.