Fig. 3: Tumor-derived pericytes display defective blood vessel supporting functions in vitro and in vivo. | Nature Communications

Fig. 3: Tumor-derived pericytes display defective blood vessel supporting functions in vitro and in vivo.

From: Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities

Fig. 3

a CCK8 proliferation assay showed that exposure with conditioned medium from NSCLC/HCC-derived NPC or TPC significantly enhanced the proliferation of HUVEC as compared to untreated HUVEC (n = 5 experimental repeats). b Wound healing assay of the HUVEC after exposed with condition medium from NSCLC/HCC-derived NPC or TPC. Representative pictures of wound healing assay in each group are given (n = 3 independent experiments). c HUVEC were either cultured alone or co-cultured with CFSE (green fluorescent dye) labeled NSCLC-derived-NPC/-TPC, fibroblast or smooth muscle cells (SMC) in a tube formation assay. d–f Bar charts show the relative total tube length, branch points and number in each group (n = 3 independent experiments). g Representative images of the serial sections of matrix plugs after immunostained with CD34, PDGFRβ, or collagen IV antibody in each group are given. h–k Violin plots show the quantification of the total number of blood vessels, lumen diameter, collagen IV level and percentage of coverage in each group (n = 3 mice per group). Results are given as means ± SEM. a Two-way ANOVA. b, d–f, h–k One-way ANOVA. Scale bars in b represents 100 μm, c 200 μm, g 50 μm.

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