Fig. 1: Lack of Rictor is lethal and causes systemic HSV-1 infection.

a Schematics of the experiment showing conditional knockout of the iRic−/− mice using Tamoxifen or mock. The mice were infected with HSV-1 McKrae and analyzed for systemic infection. b Survival curve showing percent survival after iRic+/+ and −/− infection (n = 10). Log-rank (Mantel-core) test was used to analyze the survival curve data. c Plaque assay showing mature virus particle formation in eyewash samples collected at 4 dpi (n = 11). d Representative eye image of HSV-1 infected animals at 4 dpi. e Graph showing image score based on visual observation at 6 dpi (n = 7). f Graph representing percent weight loss at 12 dpi (n = 5). g qRTPCR analysis of early viral gene isolated from blood cells at 5 dpi (n = 5). h qRTPCR analysis of late viral gene isolated from trigeminal ganglion (TG) cells at 5 dpi (n = 6). i To reactivate the virus in TG of infected mice, TG were cultured in vivo and the mature virus particles were analyzed with plaque assay (n = 3). j A representative micrograph of immunohistochemistry analysis for HSV-1 detection in brain stem at 10 dpi. k Graph representing mature virus particle formation in brain stem at 5 dpi (n = 5). Two-tailed unpaired t-test was used to analyze the data presented in c, e–i, and k. Data are represented as mean ± SEM in c, e–i, and k. Data in the micrograph (d, j) is representative of five independent experiments. Source data underlying Fig. 1b are provided as a Source Data file.