Fig. 1: Compound screening for enhancers of Kcc2 expression in primary cortical neurons yields Kenpaullone.

Screening paradigm using three rounds of primary screen based on luciferase (LUC) activity followed by secondary screen including Kcc2 RT-qPCR and clomeleon chloride imaging. Bottom panel: Four compound “winners” including Kenpaullone (KP); screening conducted in primary mouse neurons.