Fig. 3: Mof heterozygous mice are resistant to HFD-induced fat mass gain.

a Representative images of male control and Mof^+/− mice after 26 weeks of HFD. See also Supplementary Fig. 4a–d for female. b Body weight gain under standard (SD) and high-fat diets (HFD). Statistical analysis performed by two-way ANOVA, ****p = 10⁻¹⁶. SD Mof^+/+ n = 5, SD Mof^+/− n = 4, HFD Mof^+/+ n = 5, HFD Mof^+/− n = 5. Error bars indicate ±SEM. See also: Supplementary Fig. 4d. c Overall correlation between MOF trimmed mean of M (TMM) values normalized mRNA¹¹⁵ levels and body mass index (BMI). Correlation score and p value calculated by Pearson. Original data from GSE50244. d Blood glucose levels before and after OGTT (Glucose bolus 1 g/kg mouse). Assay performed after 1, 8, and 26 weeks of HFD treatment in Mof^+/+ (black) and Mof^+/− (green) mice (n = 5/group). Error bars indicate ±SEM. Statistical analysis performed by one-way ANOVA, followed by Tukey test. Significant (p < 0.05) p value is indicated in the figure. See also: Supplementary Fig. 4f. e Glycemic peak, represented as a ratio of peak glucose level (15 min after oral administration) relative to steady-state (fasting) level. Error bars indicate ±SEM. Statistical analysis performed by one-way ANOVA, followed by Tukey test *p = 0.04. n = 5/group. f Peak serum insulin levels (15 min after oral glucose administration) relative to steady state (fasting) levels. Error bars indicate ±SEM. Statistical analysis performed by one-way ANOVA, followed by Tukey test *p = 0.02 n = 5/group. g Percentage of glycated hemoglobin (HbA1c) in SD (Mof^+/+ n = 7; Mof^+/− n = 5) or HFD (n = 5/group). Error bars indicate ±SEM. Statistical analysis performed by one-way ANOVA followed by Tukey test, *p = 0.02, ***p = 0.0004. h Free water in SD or HFD as a percentage of total body composition (n = 5/group). Statistical analysis performed as in (g). Statistical analysis performed by two-sided one-way ANOVA followed by Tukey test, *p = 0.04, **p = 0.006. Error bars indicate ±SEM. See also: Supplementary Fig. 4.