Fig. 3: ntrB mutants possess comparable motility phenotypes. | Nature Communications

Fig. 3: ntrB mutants possess comparable motility phenotypes.

From: A mutational hotspot that determines highly repeatable evolution can be built and broken by silent genetic changes

Fig. 3

Surface area of motile zones within an AR2 genetic background following 48 h of growth across four environmental conditions for mutants ntrB A289C, ntrB Δ406-417, ntrB A683C and glnK Δ84; rpoN A688C. Lysogeny broth (LB, N = 9, 9, 5, 4 respective independent biological replicates), M9 minimal media with ammonia (M9, N = 7, 6, 2, 5), and M9 supplemented with amino acids glutamate (M9 + glu, N = 5, 5, 5, 4) or glutamine (M9 + gln, N = 5, 5, 5, 6) were used as nutritional conditions. Individual data points from biological replicates are plotted and each migration area has been standardised against the surface area of an AR2 ntrB A289C mutant grown in the same environment (ntrB A289C growth mean = 0). Significance values for glnK Δ84; rpoN A688C: M9 P = 0.000708,M9 + glu P = 0.0025, M9 + gln P = 0.032, LB P = 0.0048 (Kruskal–Wallis post-hoc Dunn test, one-sided). Source data are provided as a Source Data file.

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