Fig. 4: A synonymous sequence mutant (AR2-sm) confers a loss of repeatable evolution. | Nature Communications

Fig. 4: A synonymous sequence mutant (AR2-sm) confers a loss of repeatable evolution.

From: A mutational hotspot that determines highly repeatable evolution can be built and broken by silent genetic changes

Fig. 4

A Histogram of motility phenotype emergence times across independent replicates of immotile SBW25 (AR2, grey) and an AR2 strain with six synonymous substitutions in the ntrB locus (AR2-sm, white) in two nutrient conditions. Lines that evolved after the experiment timeframe (10 days) were pooled into bin 11 + . B Observed mutational targets following directed evolution of synonymous variants, performed across two environments. Each unique mutation is highlighted by an identifiable colour. (Sample sizes (N): AR2: LB N = 5, M9 N = 24; AR2-sm: LB N = 8, M9 N = 8). Note that characterised genotypes were sampled within 8 days of experiment start date. Unidentified mutations could not be distinguished from wild type sequences of genes belonging to the nitrogen regulatory pathway (ntrB, glnK and glnA) which were analysed by Sanger sequencing. ntrB Δ406-417 was the only mutational target shared by both lines within the same nutritional environment. Source data are provided as a Source Data file.

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