Fig. 6: PARP1-BRCT mutants with changed folding properties identified from a randomly generated mutant library using the dual-reporter system.
From: A dual-reporter system for investigating and optimizing protein translation and folding in E. coli
a Correlation between translation levels of 20 PARP1-BRCT mutants quantified from Western blots (gray, n = 1) and flow cytometry analysis of mean mCherry fluorescence values ± standard deviation (red), each normalized to the WT signal (n ≥ 3, biologically independent samples). b GFP levels analyzed using flow cytometry as a measure for protein solubility and folding properties. Data are presented as mean values ± standard deviation for n ≥ 3 biologically independent samples. c Percentage of soluble protein determined by Western blot for the 9 PARP1-BRCT mutants with a detectable GFP response signal. Western blot analysis of total protein yield (tot) and soluble protein (sol) after fractionated cell disruption (n = 1). Source data are provided as a Source Data file.
