Fig. 3: Ca2+ permeation through MCA1(1-173) and MCA2(1-173) reconstituted into liposomes containing Fluo-4. | Nature Communications

Fig. 3: Ca2+ permeation through MCA1(1-173) and MCA2(1-173) reconstituted into liposomes containing Fluo-4.

From: MCAs in Arabidopsis are Ca2+-permeable mechanosensitive channels inherently sensitive to membrane tension

Fig. 3

a Fluorescence from liposomes reconstituted with MCA1(1-173)-6H or MCA2(1-173)-6H or from control liposomes. Liposomes were preloaded with Fluo-4. CaCl2 was sequentially added to the bath solution containing 1 mM EGTA. A detergent was added at the end of the experiment and fluorescence at this point was used to normalize fluorescence. b Fluorescence from liposomes reconstituted with MCA1(1-173)D21N-6H or MCA2(1-173)D21N-6H. Ca2+ and a detergent were added as described above. The mean and standard deviation of three measurements. In a, the p-values of the two-sided t-test between the data from MCA1(1-173)-6H or MCA2(1-173)-6H and those from control liposomes are indicated at 2.5 mM CaCl2.

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