Fig. 5: Cdc42 is activated downstream of Giα family G proteins. | Nature Communications

Fig. 5: Cdc42 is activated downstream of Giα family G proteins.

From: Optogenetic control of receptors reveals distinct roles for actin- and Cdc42-dependent negative signals in chemotactic signal processing

Fig. 5: Cdc42 is activated downstream of Giα family G proteins.The alternative text for this image may have been generated using AI.

a Schematic indicating the potential for Cdc42 response regulation independent of heterotrimeric G protein signals. b, c Single-pulse stimulations of populations of PLB-985 cells expressing parapinopsina and the Cdc42-TomKat sensor either without (b) or after treatment with 600 ng/ml Pertussis toxin (PTX) to inhibit Giα-dependent signals (c). b Data are presented as mean ± s.e.m of nwell replicates = 16 for all conditions. c Data are presented as mean ± s.e.m (nwell replicates = 27 for relative light intensity = 0, nwell replicates = 25 for relative light intensity = 5, nwell replicates = 26 for relative light intensity = 10, nwell replicates = 16 for relative light intensity = 20, and nwell replicates = 16 for relative light intensity = 50). Stimulus duration is indicated by the gray bar. Source data are provided as a Source Data file.

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