Fig. 1: Pyramidal cell properties remain similar across bilateral CA1 after silencing CA3.

a Schematic representations of experimental protocols. Top: microdrive placement for bilateral CA1 recording; center: timeline of Dox food withdrawal, surgery, and recording; bottom: structure of the recording day. b Representative examples of firing rate maps on the linear track from the left and right CA1 of controls (CTRs) and mutants (MUTs). Colors are scaled to peak firing rate (Hz) indicated at the top of each map (blue, minimum; yellow, maximum). c Mean firing rate during exploration (CTR, N = 4 animals, n = 273 cells; MUT, N = 5 animals, n = 318 cells; P (mean firing rate during exploration) = 0.1939), rest and within SWRs was comparable between genotypes (CTR, N = 4 animals, n = 227 cells; MUT, N = 5 animals, n = 290 cells; P (mean firing rate during rest) = 0.1167; P (mean firing rate during SWRs) = 0.6285). Spatial information during exploration was significantly lower in mutants (CTR, N = 4 animals, n = 273 cells; MUT, N = 5 animals, n = 318 cells; P = 0.0046). d Pyramidal cell properties were comparable between the left and right CA1 (filled bars: left hemisphere; open bars: right hemisphere; CTR, N = 4 animals, n (left) = 152 cells, n (right) = 121 cells; MUT, N = 5 animals, n (left) = 162 cells, n (right) = 156 cells; mean firing rate during exploration: P (CTR) = 0.6816, P (MUT) = 0.1030; spatial information during exploration: P (CTR) = 0.8559, P (MUT) = 0.5042; during sleep: CTR, N = 4 animals, n (left) = 126 cells, n (right) = 101 cells; MUT, N = 5 animals, n (left) = 158 cells, n (right) = 132 cells; mean firing rate during rest: P (CTR) = 0.5773, P (MUT) = 0.5291; mean firing rate during ripple: P (CTR) = 0.2369, P (MUT) = 0.0676); significant difference in Fig. 1d were tested with LMMs. Unless mentioned, all significant difference in Fig. 1 were tested with two-sided Wilcoxon’s rank-sum test.