Table 2 Peptide sequences and IC₅₀ values from phosphopeptide competition assay.

Protein	Phosphotyrosine	Sequence															IC₅₀ (nM)^a
		−7	−6	−5	−4	−3	−2	−1	0	+1	+2	+3	+4	+5	+6	+7
JAK2	pY813				F	T	P	D	pY	E	L	L	T	E	N	D	138
	pY1007/pY1008	A	I	E	T	D	K	E	pY	pY	K	V	K	E	P	G	>10,000
	pY1007	A	I	E	T	D	K	E	pY	Y	K	V	K	E	P	G	>10,000
	pY1008	I	E	T	D	K	E	Y	pY	K	V	K	E	P	G		>10,000
JAK3	pY785				I	S	S	D	pY	E	L	L	S	D	P	T	305
IRK	pY1445/pY1446					E	T	D	pY	pY	R	K	G	G	K	G	>10,000
	pY521				F	P	A	H	pY	R	R	L	R	H	A	W	>10,000
	pY552				V	L	G	Q	pY	L	R	D	T	A	A	L	>10,000
MPL	pY591				A	Q	D	M	pY	R	R	L	Q	P	S	C	>10,000
	pY626				A	N	H	S	pY	L	P	L	S	Y	W	Q	4887
	pY631				L	P	L	S	pY	W	Q	Q	P				>10,000
	pY309				Q	L	W	L	pY	Q	N	G	C	L	W	W	>10,000
	pY368				A	Q	D	T	pY	L	V	L	D	K	W	L	1121
EPOR	pY426				A	S	F	E	pY	T	I	L	D	P	S	S	1557
	pY454				P	H	L	K	pY	L	Y	L	V	V	S		661
	pY485				S	D	G	P	pY	S	N	P	Y	E	N	S	>10,000
FLT3	pY572		Y	K	K	Q	F	R	pY	E	S	Q	L	Q	M		>10,000
	pY591		S	D	N	E	Y	F	pY	V	D	F	R	E	Y		1250
	pY919		Y	A	T	E	E	I	pY	I	I	M	Q	S	C		1550
PDGFR	pY572					G	H	E	pY	I	Y	V	D	P			3810
c-KIT	pY568					G	N	N	pY	V	Y	I	D	P			1935
c-FMS	pY923					E	R	D	pY	T	N	L	P	S			>10,000

Target phosphotyrosine and mutated residues are highlighted in bold. Data are given as the mean of at least technical two replicates resulting from two, three or four independent experiments.
^aIC₅₀ as determined by SPR competition assay.

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