Fig. 3: Characterization of bone marrow-derived JAK2V617F hematopoietic cells in the lungs by the use of GFP-transgene.

a Lethally irradiated WT mice were transplanted with bone marrow (BM) cells from control WT/CAG-EGFP (WT-GFP) or JAK2^V617F/CAG-EGFP (JAK2^V617F-GFP) double transgenic mice. Five weeks after BM transplantation (BMT), the recipient mice were subjected to chronic hypoxia for 3 weeks, and then the lungs were fixed and stained with the indicated antibodies. Representative immunofluorescence images of lung sections stained with anti-GFP (green) and anti-αSMA (red) antibodies and DAPI (blue) in WT-GFP-BMT or JAK2^V617F-GFP-BMT mice. The boxed areas from JAK2^V617F-GFP-BMT mice at higher magnifications (high) are shown in the bottom panels. Scale bars, 100 µm. b Quantitative analysis of Ly6G⁺ cells in the perivascular regions (n = 3 in each group, ^*P = 0.0223). c Representative immunofluorescence images of lung sections stained with anti-GFP (green) and anti-Ly6G (red) antibodies, as well as DAPI (blue) in WT-GFP-BMT or JAK2^V617F-GFP-BMT mice. The boxed areas from JAK2^V617F-GFP-BMT mice at higher magnifications are shown in the bottom panels (high). Scale bars, 100 µm. Quantitative analysis of Ly6G-exressing cells in GFP⁺ cells (d, n = 3 in each group, ^*P = 0.0052) and GFP-expressing cells in Ly6G⁺ cells (e, n = 3 in each group). More than 100 GFP⁺ cells and Ly6G⁺ cells were counted in each section and expressed as the percentage of the cells. All data are presented as mean ± SEM. WT-GFP-BMT, recipient WT mice transplanted with WT-GFP BM cells; JAK2^V617F-GFP-BMT, recipient WT mice transplanted with JAK2^V617F-GFP BM cells. ^*P < 0.05 versus WT-GFP recipients by the unpaired t test (two-sided). Source data are provided as a Source Data file.