Fig. 1: 3D light-sheet imaging of the murine calvarium at single-cell resolution.
From: Quantitative 3D imaging of the cranial microvascular environment at single-cell resolution
A Maximum intensity projection (MIP) of vessels and Osterix+ skeletal progenitors in the parietal and posterior frontal bones of the calvarium. B Images from the boxed region in A demonstrating the high resolution and signal quality obtained in each channel (i–iv). The dyes used for each channel are designated above (A) and (B): AF555 for Endomucin (i), AF647 plus for Osterix (ii), and AF800 plus for CD31 (iii). C, D Sagittal (C) and frontal (D) 40-μm-thick optical sections demonstrating the location of vessels and Osterix+ cells along the thickness of the calvarium. Boxed regions from (Ci) and (Di) are shown in Cii and Dii. Similar results were achieved for all the calvaria imaged in this study (n = 45 calvaria). Scale bars: 1000 μm (A, Di), 800 μm (Ci), 300 μm (Cii), 100 μm (Bi–iv, Dii). Colors: Red: Endomucin (Emcn), Gray: Osterix, Green: CD31.
