Fig. 3: Correlation of aptamer and antibody binding.
From: A comparative analysis of cell surface targeting aptamers
The median fluorescence staining of all aptamers at 500 nM are shown relative to the median fluorescence of C36 for each cell type. Each aptamer is compared to a commercially available antibody against the aptamer’s reported target using either the binding (red) incubated at room temperature or internalization and binding with blocking (green) protocol incubated at 37 °C or internalization and binding without blocking (purple) incubated at 37 °C. Antibody signals are shown relative to an isotype control on each cell line (blue). Aptamers A C2.min and B Waz compared to hTfR antibody. Aptamers C A9.min, D A10-3, and E A10-3.2 compared to PSMA antibody. Aptamers F E07 and G CL4 compared to EGFR antibody. Aptamer H GL21.T compared to AXL antibody. Aptamers I SE15-8-mini and J 2-2(t) compared to HER2 antibody. Aptamer K EpDT3 compared to EpCAM antibody. Data represent the mean of three independent experimental replicates for antibodies and four independent experimental replicates for aptamers ±SD. Source data are provided as a Source Data file.
