Fig. 5: In vivo imaging and validation of aptamer function.

Nude mice bearing subcutaneous xenograft tumors on their right flank were injected with 2 nmol of AF750 labeled aptamer (A). Dorsal and lateral images of mice bearing 22Rv1 tumors 12 h post injection. The location of the tumor is circled in purple. Injected aptamers are as indicated. Three animals from each cohort (n = 6) are shown. Additional animals in the cohorts can be found in Supplementary Fig. 188. A time course for a subset of animals (n = 3) used in this experiment can be found in Supplementary Fig. 189. B Dorsal and lateral images of mice bearing PC3-PSMA tumors 12 h post injection. The location of the tumor is circled in purple. Injected aptamers are as indicated (n = 3). A time course for a subset of animals used in this experiment can be found in Supplementary Fig. 190. C Predicted secondary structure of Waz and location of base mutations used to generate mutant variants Waz X and Waz GGG. D Relative binding affinity of Waz, Waz.X and Waz.GGG on 22Rv1 cells. E Dorsal and lateral images of mice bearing 22Rv1 tumors 12 h post injection. The location of the tumor is circled in purple. F Quantitation of fluorescence signal within tumors following injection with Waz, Waz.X, Waz.GGG, or C36 (t = 12 h, n = 3). Data represents the mean ± SD. Source data are provided as a Source Data file.