Table 3 Correlative analysis of aptamer and antibody binding.

		Binding		Internalization with blocking		Internalization without blocking
Target	Aptamer	ρ	P value	ρ	P value	ρ	P value
hTfR	C2	0.67	0.0277	0.72	0.0162	0.60	0.0562
hTfR	Waz	0.62	0.0478	0.65	0.0368	0.58	0.0656
PSMA	A9	0.68	0.0251	0.83	0.0027	0.79	0.0055
PSMA	A10-3	0.35	0.2993	−0.02	0.9673	0.30	0.3713
PSMA	A10-3.2	0.01	0.9895	−0.35	0.2862	0.03	0.9462
EGFR	E07.min	0.88	0.0007	0.85	0.0018	0.95	>0.0001
EGFR	CL4	−0.03	0.9462	0.04	0.9241	0.28	0.4023
HER2	SE15-8-mini	0.35	0.2862	0.02	0.9673	−0.61	0.0519
HER2	2-2	0.16	0.6337	−0.08	0.8179	−0.47	0.1457
EpCAM	EpDT3	−0.37	0.2608	−0.33	0.3269	−0.10	0.7759
AXL	GL21.T	0.05	0.9033	0.40	0.2250	0.39	0.2366

Median fluorescence of each aptamer relative to non-specific control, C36, was compared to the median fluorescence of a commercially available antibody against the same receptor relative to an isotype control using a Spearman Correlation with a two tailed P value. This was performed for all aptamers using our three different assay conditions: binding, Internalization, and binding with blocking (blocked), and internalization and binding without blocking (unblocked). Significant correlation was defined for P < 0.05 and are represented in bold.
ρ Spearman’s correlation coefficient.

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