Table 1 Michaelis–Menten parameters from lysate kinetic data.

From: Improvement of a synthetic live bacterial therapeutic for phenylketonuria with biosensor-enabled enzyme engineering

Strain

StlA variant

Vmax (μmol TCA/min)

KM (mM Phe)

EP2315

wild-type

3.20 ± 0.63

1.69 + 0.37

EP2516

S92G_H133F_A433S_V470A

11.96 ± 1.64

4.06 + 0.44

EP2525

S92G_H133M_I167K_L432I_V470A

10.70 ± 1.89

4.40 + 0.27

  1. Vmax and KM average of n = 3 biological replicates ±s.d.
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