Fig. 1: Map-based cloning of the Rps11 locus.

a Comparison of the NLR gene clusters between Williams 82 (assembly V3.0) and PI 594527. Black boxes represent NLR genes and light-blue shades represent syntenic blocks between two genomes. b Physical locations of the markers used for fine mapping of the Rps11 locus. The final mapping boundaries are highlighted by orange dashed lines. c Expression analyses in key recombinants by RNA-seq. Green/light-green boxes represent the five expressed NLR gene from PI 594527, black boxes represent the non-expressed NLR genes from PI 594527. Gray boxes represent the NLR genes from Williams 82. Dashed lines represent unknown genotype. The phenotype of each key recombinant is indicated as resistant or susceptible at the right side of each recombinant type. d Relative expression of R6 in different tissues. Values were shown as mean ±  SEs of the means from three biological replicates normalized to expression of R6 in the root sample, which was set as 1.0. e 5′-Rapid amplification of cDNA ends (5′-RACE) performed for the 5 expressed NLR genes (R1, R4, R6, R9, and R12) to determine transcription state region (TSR). x axis represents the distance upstream of the first exon. Brown bars represents the 5′-RACE reads mapped to each NLR gene. Orange lines/shades show the promoter regions sharing sequence similarity. Arrows at the right side indicate the direction of the NLR genes. f Gene model of Rps11 (R6). The vertical arrow points to the TSR. Orange color indicates the region encoding the NBS domain; light-blue color indicates the regions encoding the LRR domains; gray color indicates the region without a domain detected. Open boxes represent genomic fragments corresponding to 5′- and 3′-UTRs. Source data underlying Fig. 1d are provided as a Source Data file.