Fig. 3: Sp7 controls the expression of genes involved in neuron projection development in osteocytes.
From: Control of osteocyte dendrite formation by Sp7 and its target gene osteocrin
a RNA-seq was performed on control (shLacZ) and Sp7 knockdown (KD, top) and control (LV-GFP) and Sp7 overexpressing (OE, bottom) Ocy454 cells. Volcano plots demonstrate differentially expressed genes (red data points) in each comparison. See Supplementary Data 2. b Gene ontology analysis of genes downregulated by Sp7 knockdown (top) and upregulated by Sp7 overexpression (bottom) reveals enrichment in several terms associated with cell projection development and neuronal morphogenesis. Fisher’s exact test with correction for multiple comparisons was used to determine the significance of enriched gene ontology terms. c–e Relationship between gene expression changes in response to Sp7 perturbation. Top, Venn diagram revealing number of genes regulated by both Sp7 overexpression and Sp7 knockdown. Middle, scatterplot showing fold change regulation of all detected genes by Sp7 knockdown (x-axis) and Sp7 overexpression (y axis). Bottom, RRHO2 visualization reveals statistically significant groups of genes counter-regulated by Sp7 knockdown versus overexpression. f Heatmap showing fold change regulation of individual genes in gene ontology groups of interest (neuron cell projection and axon guidance).
