Fig. 1: Reactions of C-deglycosylation enzymes.

a Two-step C-C bond cleavage reaction by oxidoreductase (DgpA) and C-deglycosylation enzymes (DgpC and DgpB; PuCGD). b C-deglycosylation enzymes used in our studies: PuCGD, EuCGD, MiCGD, AgCGD1, AgCGD2, and MtCGD. Sugar moieties are shown in red. The substrates investigated in this study were C8-glycosylated flavonoids 1 and 2, C6-glycosylated flavonoids 3 and 4, C-glycosylated xanthonoid 5, and C-glycosylated anthraquinone 6.