Fig. 6: Mutagenesis analysis of PuCGD^α and MiCGD^α.

The numbers in brackets represent the corresponding numbers of the residues in AgCGD2. All experiments were repeated independently more than three times, and similar results were obtained. Statistical comparisons between means for the wild type (WT) and each mutant were performed by Student’s t-test (2-tailed). The p-values were shown above each bar. The bars are means of n = 3 independent experiments (each data point was indicated by a black circle) and error bars indicate standard deviations. Data are presented as mean values + /− SD. All experiments were repeated independently three times with similar results.